【Abstract】 Objective To study the practicability of double antigens sandwich enzyme linked immunosorbent assay(DAgS?ELISA)on the detection of Yersinia pestis F1 antibodies. Methods A total of 558 samples antibodies of anti?F1 antigen were detected by DAgS?ELISA and trace indirect hemagglutination assay (trace?IHA). Results Thirty three samples were positive tested by IHA, 31 positive by DAgS?ELISA, the positive accordance rate was 90.91%, 99.81% for negative accordance rate, 99.28% for the total accordance rate. The positive rate detected by IHA and DAgS?ELISA were 5.91% and 5.56% respectively, and no statistics difference was found (χ2=0.25,P=0.625). About 27 the immuno?serum were positive detected by IHA and DAgS?ELISA methods, and the sensitivity of IHA test were all higher than that of DAgS?ELISA (t=3.023, P=0.006). Conclusion Sensitivity of DAgS?ELISA is lower than that of trace?IHA, but its specificity is better and no primary inhibitory phenomena, and could exempt from leak detection in the preliminary screening.
2009, 20 (5):
467-469.